Review




Structured Review

STEMCELL Technologies Inc sepmate pbmc isolation tubes
Sepmate Pbmc Isolation Tubes, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sepmate+pbmc+isolation+tubes/sepmate+pbmc+isolation+tubes/bio_rxiv__2025__07__15__664896-65-19-23
Average 90 stars, based on 1 article reviews
sepmate pbmc isolation tubes - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Gradient Centrifugation:

Article Title: A New Method of Canine CD4 + T Lymphocyte Differentiation Towards the Th17 Phenotype with Analysis of Properties and Mitochondrial Activity.
Article Snippet: The anticoagulated blood was diluted with sterile PBS in a ratio 1:2 prior administration to a density gradient medium (Lymphoprep, Stemcell Technologies, Vancouver, BC, Canada) that was used to isolate canine peripheral blood mononuclear cells (PBMCs). .. Density gradient centrifugation was performed in SepMate PBMC isolation tubes (Stemcell Technologies, Canada) at 800× g, 10 min, at room temperature (RT). .. Afterwards, the isolated cells were washed twice with PBS supplemented with 2mM EDTA and 2% FBS and treated with erythrocyte lysis buffer (ACK lysing buffer, Thermo Fisher Scientific, Waltham, MA, USA) for 4 min at RT.

Article Title: A New Method of Canine CD4 + T Lymphocyte Differentiation Towards the Th17 Phenotype with Analysis of Properties and Mitochondrial Activity
Article Snippet: The anticoagulated blood was diluted with sterile PBS in a ratio 1:2 prior administration to a density gradient medium (Lymphoprep, Stemcell Technologies, Vancouver, BC, Canada) that was used to isolate canine peripheral blood mononuclear cells (PBMCs). .. Density gradient centrifugation was performed in SepMate PBMC isolation tubes (Stemcell Technologies, Canada) at 800× g , 10 min, at room temperature (RT). .. Afterwards, the isolated cells were washed twice with PBS supplemented with 2mM EDTA and 2% FBS and treated with erythrocyte lysis buffer (ACK lysing buffer, Thermo Fisher Scientific, Waltham, MA, USA) for 4 min at RT.

Isolation:

Article Title: A New Method of Canine CD4 + T Lymphocyte Differentiation Towards the Th17 Phenotype with Analysis of Properties and Mitochondrial Activity.
Article Snippet: The anticoagulated blood was diluted with sterile PBS in a ratio 1:2 prior administration to a density gradient medium (Lymphoprep, Stemcell Technologies, Vancouver, BC, Canada) that was used to isolate canine peripheral blood mononuclear cells (PBMCs). .. Density gradient centrifugation was performed in SepMate PBMC isolation tubes (Stemcell Technologies, Canada) at 800× g, 10 min, at room temperature (RT). .. Afterwards, the isolated cells were washed twice with PBS supplemented with 2mM EDTA and 2% FBS and treated with erythrocyte lysis buffer (ACK lysing buffer, Thermo Fisher Scientific, Waltham, MA, USA) for 4 min at RT.

Article Title: Development of a First-in-Class Click Chemistry–Based Cancer Therapeutic, from Preclinical Evaluation to a First-in-Human Dose Escalation Clinical Trial
Article Snippet: The data were acquired and integrated by the data acquisition system Analyst TF software (SCIEX, RRID: SCR_015785) linked directly to the API 4000 MS/MS detector and then processed in Watson LIMS (Thermo Fisher Scientific). .. Immune cell profiling of patient samples by cytometry by time of flight Peripheral blood mononuclear cells (PBMC) were isolated from patient whole blood samples using SepMate PBMC isolation tubes (STEMCELL Technologies) and Lymphoprep (STEMCELL Technologies) according to the manufacturer’s instructions. .. Isolated PBMC were frozen in chilled CryoStor CS10 freezing media (STEMCELL Technologies).

Article Title: Hematological Changes and Immunomodulation of Neutrophil and Monocyte Populations in Shelter Dogs.
Article Snippet: For cPBMCs isolation, 6 mL of blood was diluted with sterile PBS at a 1:2 ratio and layered onto a density gradient medium (Lymphoprep, Stemcell Technologies, Vancouver, Canada). .. Centrifugation was performed using SepMate PBMC isolation tubes (Stemcell Technologies, Vancouver, Canada) at 800× g, 10 min, at room temperature (RT). ..

Article Title: Dual Role of Glycosylation in Resistance to CD4-binding Site Broadly Neutralizing Antibodies
Article Snippet: .. Uninfected, target cells were isolated from buffy coat obtained from GulfCoast Blood Bank (Houston, TX) by isolating PBMC using SepMate PBMC Isolation tubes (STEMCell, Vancouver, BC) and CD8-depleting these cells with CD8 + Dynabeads (ThermoFischer Scientific, Waltham, MA) according to the manufacturer’s instructions. ..

Article Title: The gut microbiota promotes pain in fibromyalgia.
Article Snippet: A 4.5 ml Ficoll Paque Plus solution (Cat No. GE17-1440-02, Sigma Aldrich) was added beneath the diluted blood, and tubes were centrifuged at 400 x g. The buffy coat layer was harvested and rinsed with the balanced salt solution (3X the volume of the buffy coat layer). .. Peripheral blood mononuclear cell (PBMCs) collection Blood (200 ml) was collected from the cheek vein into EDTA-coated tubes, diluted 1:3 with a balanced salt solution (1X Dulbecco0s PBS (DPBS, Cat No. TMS-012-A Sigma Aldrich) fortified with 2% FBS (Cat No. F2442, Sigma Aldrich)), and transferred to 15 ml SepMate PBMC isolation tubes (Cat No. 85415, STEMCELL Technologies). .. A 4.5 ml Ficoll Paque Plus solution (Cat No. GE17-1440-02, Sigma Aldrich) was added beneath the diluted blood, and tubes were centrifuged at 400 x g. The buffy coat layer was harvested and rinsed with the balanced salt solution (3X the volume of the buffy coat layer).

Article Title: Tumor-derived erythropoietin acts as an immunosuppressive switch in cancer immunity
Article Snippet: .. Fresh human PBMCs were isolated from whole blood using SepMate PBMC Isolation Tubes (StemCell Technologies). .. PBMCs were cultured in ImmunoCultTM-SF Macrophage Differentiation Medium supplemented with 50 ng/ml of human recombinant M-CSF (PeproTech).

Article Title: A New Method of Canine CD4 + T Lymphocyte Differentiation Towards the Th17 Phenotype with Analysis of Properties and Mitochondrial Activity
Article Snippet: The anticoagulated blood was diluted with sterile PBS in a ratio 1:2 prior administration to a density gradient medium (Lymphoprep, Stemcell Technologies, Vancouver, BC, Canada) that was used to isolate canine peripheral blood mononuclear cells (PBMCs). .. Density gradient centrifugation was performed in SepMate PBMC isolation tubes (Stemcell Technologies, Canada) at 800× g , 10 min, at room temperature (RT). .. Afterwards, the isolated cells were washed twice with PBS supplemented with 2mM EDTA and 2% FBS and treated with erythrocyte lysis buffer (ACK lysing buffer, Thermo Fisher Scientific, Waltham, MA, USA) for 4 min at RT.

Article Title: Deep metabolic profiling of immune cells by spectral flow cytometry—A comprehensive validation approach
Article Snippet: .. PBMC was isolated using the SepMate PBMC Isolation Tubes (StemCell Technologies), according to the manufacturer’s instructions. .. Briefly, blood was diluted 1:1 with PBS (15 mL each) and carefully layered over Lymphoprep density gradient medium (StemCell Technologies) in a SepMate tube and centrifuged for 1200 x g for 10 min at room temperature with brakes.

Cytometry:

Article Title: Development of a First-in-Class Click Chemistry–Based Cancer Therapeutic, from Preclinical Evaluation to a First-in-Human Dose Escalation Clinical Trial
Article Snippet: The data were acquired and integrated by the data acquisition system Analyst TF software (SCIEX, RRID: SCR_015785) linked directly to the API 4000 MS/MS detector and then processed in Watson LIMS (Thermo Fisher Scientific). .. Immune cell profiling of patient samples by cytometry by time of flight Peripheral blood mononuclear cells (PBMC) were isolated from patient whole blood samples using SepMate PBMC isolation tubes (STEMCELL Technologies) and Lymphoprep (STEMCELL Technologies) according to the manufacturer’s instructions. .. Isolated PBMC were frozen in chilled CryoStor CS10 freezing media (STEMCELL Technologies).

Centrifugation:

Article Title: Hematological Changes and Immunomodulation of Neutrophil and Monocyte Populations in Shelter Dogs.
Article Snippet: For cPBMCs isolation, 6 mL of blood was diluted with sterile PBS at a 1:2 ratio and layered onto a density gradient medium (Lymphoprep, Stemcell Technologies, Vancouver, Canada). .. Centrifugation was performed using SepMate PBMC isolation tubes (Stemcell Technologies, Vancouver, Canada) at 800× g, 10 min, at room temperature (RT). ..



Similar Products

90
STEMCELL Technologies Inc sepmate pbmc isolation tubes
Sepmate Pbmc Isolation Tubes, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sepmate+pbmc+isolation+tubes/sepmate+pbmc+isolation+tubes/bio_rxiv__2025__07__15__664896-65-19-23
Average 90 stars, based on 1 article reviews
sepmate pbmc isolation tubes - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc pbmc isolation via ficoll using lymphoprep-filled sepmate tubes
Pbmc Isolation Via Ficoll Using Lymphoprep Filled Sepmate Tubes, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sepmate+pbmc+isolation+tubes/sepmate+pbmc+isolation+tubes/pm40579545-251-9-14
Average 90 stars, based on 1 article reviews
pbmc isolation via ficoll using lymphoprep-filled sepmate tubes - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc sepmate pbmc isolation tube
Sepmate Pbmc Isolation Tube, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sepmate+pbmc+isolation+tubes/sepmate+pbmc+isolation+tubes/pm40373182-31-24-28
Average 90 stars, based on 1 article reviews
sepmate pbmc isolation tube - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
STEMCELL Technologies Inc sepmate-50 pbmc isolation tubes
Sepmate 50 Pbmc Isolation Tubes, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sepmate+pbmc+isolation+tubes/sepmate+tubes/pmc12021410-145-25-29
Average 90 stars, based on 1 article reviews
sepmate-50 pbmc isolation tubes - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results